View Full Version : Procyanidin B-2, Proanthocyanidins

01-27-2012, 01:06 PM
Br J Dermatol. 2002 Jan;146(1):41-51.
Procyanidin B-2, extracted from apples, promotes hair growth: a laboratory study. Kamimura A, Takahashi T.
Source: Tsukuba Research Laboratories, Kyowa Hakko Kogyo Co., 2, Miyukigaoka, Tsukuba, Ibaraki 305-0841, Japan. ayako.kamimura@kyowa.co.jp
We observed that procyanidin B-2 reduces the expression of PKC-alpha, -betaI, -betaII and -eta in cultured murine hair epithelial cells and also inhibits the translocation of these isozymes to the particulate fraction of hair epithelial cells. Our immunohistochemical analyses demonstrated that PKC-alpha, -betaI, -betaII and -eta are specifically expressed in the outer root sheaths of both anagen and telogen hair follicles. The hair matrix at the anagen stage showed no positive staining for these PKC isozymes. Moderate to intense staining for PKC-betaI and -betaII in the epidermis and hair follicles was observed in a telogen-specific manner; however, expression of PKC-alpha and -eta during the telogen stage was not conspicuous. Gö 6976, an inhibitor of calcium-dependent (conventional) PKC, proved to promote hair epithelial cell growth.

Investigation of Topical Application of Procyanidin B-2 from Apple to Identify its Potential use as a Hair Growing Agent
Authors: Kamimura A, Takahashi T, Watanabe Y
Source: Phytomedicine. 2000;7(6):529-536.

Abstract: Procyanidin B-2 is a polyphenol compound we have identified in apple which acts as a hair-growing factor in the murine model both in vitro and in vivo. This report describes our investigation of the effects of 1% procyanidin B-2 tonic on human hair growth after sequential use for 6 months. A double-blind clinical test involving a total of 29 subjects was performed. Nineteen men in the procyanidin B-2 group and 10 men in the placebo control group were subjected to analyses. No adverse side effects were observed in either group. The hair-growing effect was evaluated using a macrophotography technique combined with measurements of the hair diameter of clipped hairs. The increase in number of total hairs in the designated scalp area (0.5 cm square = 0.25 cm2 area) of procyanidin B-2 group subjects after the 6 month trial was significantly greater than that of the placebo control group subjects (procyanidin B-2, 6.68 + or = 5.53 (mean + or = SD)/0.25 cm2; placebo, 0.08 ± 4.56 (mean ± SD)/0.25 cm2; P < 0.005, two-sample t test). The increase in number of terminal hairs, which are defined as hairs more than 60 mcm in diameter, in the designated area (0.5 cm square = 0.25 cm2 area) of the procyanidin B-2 group subjects after the 6-month trial was significantly greater than that of the placebo control group subjects (procyanidin B-2, 1.99 + or = 2.58 (mean ± SD)/0.25 cm2; placebo, -0.82 + or = 3.40 (mean ± SD)/0.25 cm2; P < 0.02, two-sample t test). Procyanidin B-2 therapy shows potential as a safe and promising cure for male pattern baldness.

“Proanthocyanidins promote hair follicle cell proliferation and the anagen phase of hair growth.”
Takahashi T, Kamiya T, Yokoo Y. Acta Derm Venereol (Stockh) 78: 428-432, 1998.

In recently published research, Takahashi et al examined 1000 different plant products to determine if any of them could influence hair growth. They determined that proanthocyanidins extracted from grape seeds promoted the proliferation of hair cells by 230%. They also determined that proanthocyanidins converted the telogen (non-growing) phase of hair growth into the anagen (growing) phase of hair growth [1]. In this experiment, proanthocyanidins displayed hair-cycle-converting activity which was similar to that of minoxidil. At the end of their report, the authors say that “We are now investigating the possibility of the use of proanthocyanidins as agents for curing androgenic alopecia.”

01-27-2012, 01:09 PM
"In this study, authors from the Tsukuba Research Laboratories and the Watanabe Dermatological Clinic in Japan investigated the hair growth and side effects of a 1 percent procyanidin B-2 solution extracted from commercially available apple juice.

For 6 months, 19 men received a daily topical dose of 30 mg of the procyanidin-B2 hair tonic, while another 10 received a placebo that looked and smelled the same. Hair growth--including hair density and diameter--were measured using microscopes and photographs.

Results revealed that men who received procyanidin B-2 grew more total hairs and more dense hairs than the placebo group. When the researchers compared their results with those of currently available medications, they found that procyanidin B-2 caused less hair growth than minoxidil (Rogaine), but a greater increase in total hairs than finasteride (Proscar). Unlike these prescription drugs, procyanidin B-2 produced no side effects such as itching or irritation.

The scientists speculated that antioxidants in procyanidin B-2 defeat male baldness by decreasing the scalp swelling that may cause hair loss. "The suppression of inflammation mediated by procyanidin B-2 returns the scalp to a healthy condition, consequently leading to a cure for baldness," they said. The authors are now planning further research on the length of time and the dose at which procyanidin B-2 is most effective. And they've figured out a way to get this natural chemical to bald men without using apple juice off the grocery shelf--by extracting it from green apples.


Kamimura A, Takahashi T, Watanabe Y. Investigation of topical application of procyanidin B-2 from apple to identify its potential use as a hair growing agent. Phytomedicine. 2000;7(6):529-536."

01-27-2012, 02:21 PM
what about eating apples? Would that make any difference?