View Full Version : Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells.

12-10-2015, 09:48 AM
Stem Cells Int. 2016;2016:5831276. Epub 2015 Nov 16.
Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells.

Kiratipaiboon C1, Tengamnuay P2, Chanvorachote P3.
Author information

Improvement in the expansion method of adult stem cells may augment their use in regenerative therapy. Using human dermal papilla cell line as well as primary dermal papilla cells as model systems, the present study demonstrated that ciprofloxacin treatment could prevent the loss of stemness during culture. Clonogenicity and stem cell markers of dermal papilla cells were shown to gradually decrease in the culture in a time-dependent manner. Treatment of the cells with nontoxic concentrations of ciprofloxacin could maintain both stem cell morphology and clonogenicity, as well as all stem cells markers. We found that ciprofloxacin exerted its effect through ATP-dependent tyrosine kinase/glycogen synthase kinase3β dependent mechanism which in turn upregulated β-catenin. Besides, ciprofloxacin was shown to induce epithelial-mesenchymal transition in DPCs as the transcription factors ZEB1 and Snail were significantly increased. Furthermore, the self-renewal proteins of Wnt/β-catenin pathway, namely, Nanog and Oct-4 were significantly upregulated in the ciprofloxacin-treated cells. The effects of ciprofloxacin in preserving stem cell features were confirmed in the primary dermal papilla cells directly obtained from human hair follicles. Together, these results revealed a novel application of ciprofloxacin for stem cell maintenance and provided the underlying mechanisms that are responsible for the stemness in dermal papilla cells.
PMID: 26649051 [PubMed - as supplied by publisher]

12-11-2015, 05:55 AM
I saw this yesterday and was thinking of posting it. I was assuming if it was of relevance some of the real bio-heads here would...


Is this really an effective culturing method? How does it stack up against the methods being currently used? Who was the team behind the study and what's their motive for trying to culture DPCs? Are they already making moves to transfer these to an AGA scalp?

Rashid Rashid, MD, PhD
12-11-2015, 06:37 AM
This is a facinating concent that shows how medications have multiple potential effects. It is like
-minoxidil was a blood pressure medication that was found to stimulate hair growth
-statins are cholesterol medications initially used/developed for anti inflammatory purpose http://www.ncbi.nlm.nih.gov/pubmed/20684142
-ketoconazole, well known antifungal, also stimulates hair growth by being antiandrogenic but is also antiparasitic.

Medicine and science are fascinating!

12-11-2015, 10:04 AM
I saw this the other day and was thinking of posting it, but I assumed if it was of any relevance the real bio-heads here would do so.

Questions :

How does this compare to the culturing methods currently being used by the guys working with this kind of tech?

Who was the team behind this study?

What was their motivation for doing this kind of study?

Do they have any plans for a follow up study of implanting these DPCs into human scalp in vivo?

How important is the discovery of the function of pathways they've discovered?

Ciprofloxacin has been around since 1987, why didn't someone try this earlier?