This is the picture was mentioned on the website the longitudinal bisection then why do we see follicle with bulb in Petri dish, the following links were not opening in the previous post so posting it again:-

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic
Dear Members,
I had to come to this thread because IM has quoted me and started this thread which is connected to me. I will have to defend my claim.
I hope it is fair for all to have counter argument against the title of this thread. Let discuss whether misleading claim 2 is misleading or is it true?
Preservation medium is an Illusion.
You have given your defense saying that even plucked hair of which you have shown the picture which have follicular stem cells. You are correct there is no dispute.
Counter claim:-
1. Then plucked hair can be used for doubling why to use invasive methods like hollow needles or triple way needles. Dr. Cooley, Dr. Cole have experimented with plucked hair with partial success. We have also started our experiment with addition of stem cells, dp cells, growth factor, ECM and will post the test results next month.
2. Since inherent (you have not done anything to them) stem cells are already present in a plucked follicle / in-vivo bisected follicles & in-vitro bisected follicles including FUT & FUE follicles and since you have already agreed in another thread, that the technical name of this technique is perpendicular partial longitudinal bisection of follicle in-vivo follicular doubling and hence the claims stem cells hair transplant will definitely be consider misleading.
3. The website mentions, that with the help of the needle you extract stem cells and you implant stem cells.
Prove it, which is impossible, in fact you yourself have mentioned in this post that this technique is partial longitudinal follicle bisection and implantation with its inherent stem cells.
Please prove scientifically how the preservation medium multiplies follicle and show any scientific method / article proving that by soaking the graft in any possible culture medium, GF and any other stuff, can multiply follicular stem cells. (It may help in survival and better nutrition).
By the way stem cells in the donor area are already active unlike the stem cells in the MPB recipient bald area.
4. For stem cells to activate you need to loosen the cell membrane with enzymes and special CO2 incubation in a series of process with Growth Factor and proteins in the culture media to keep its tricogensity alive and work on sodium potassium pump on the cell membrane to promote the cell division,
the real stem cell multiplication which is only possible in a FDA certified lab with high sterile conditions like number of organism per 1000 cubic cm in the air of the lab. In normal procedure room or operation theater without laminar air flow, etc one organism is sufficient to spoil the sample of stem cell which is of now use.
Unless you isolate stem cells from the surrounding cells of even the follicle one cannot activate or multiply stem cells. Confirm the same from any independent bio-tech
Next misleading claim according to you:
Let the member and experts decide whether it is a misleading claim or true. This is what you have shown on your website.

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic
That you have bisected the follicle longitudinally and extracted the follicle. But why do we see follicle with bulb in your Petri dish.
We also see transected follicles which you count as 1 instead of 2 follicle per grafts. Why 80% regeneration at donor. Why wastage of some grafts? A follicle with a bulb is like an FUE implantation and recipient will always show growth.

Next claim
You yourself claiming that you give only 80% donor regeneration that means you sacrifice 20% grafts are less at the donor after the procedure.
Now you will have to decide, as I will mention few other technique of donor doubling which will be better.
1. In-vivo hair donor doubling with partial extraction of follicle transverse or longitudinal under the vision of ultra vision or fiber optic camera with addition of GF, ECM, DP Cells, Stem cells. We promise 100% regeneration at donor and 80 to 90 % regeneration at recipient. We can convert nw7 to nw2 in 10 days, ofcourse scarless.
2. Your blind technique of extraction of partial follicular unit longitudinally with wastage of few graft, multiple extraction sites at donor and extraction of follicle with bulb wherein it should have been extraction of follicle as shown in your pictures. Why we should not consider it as a splitting of 2 follicle into 1 as shown in the petri dish. Yes both of us is scarless. you claim 80% donor regeneration and X percentage of recipient regeneration. You can repeat procedure after 6 months and nw7 will takes years to become nw2.
We also offer in-vitro donor doubling wherein we grown 90 follicles from 23 follicle.
Shortly we will also offer pluck hair transplant with addition of stem cell, DP cells, GF to improve on the findings of Dr. Cole, Dr. Cooley.
Next Claim
You yourself say, that you have to use multiple drills or multiple extraction at the donor, We do not use multiple drills or multiple extraction at donor,
Then you decide which technique will be desirable.
Kindly also have a look at different possible types of graft extraction and decide which will be better.
Dr. Nigam's FUE extracted graft

Click the below link to enlarge the above pic
Dr. Nigam's FUT extracted graft

Click the below link to enlarge the above pic
Dr. Nigam's Plucked graft

Click the below link to enlarge the above pic
Please understand we all are here to improve the present technique and get to the cure of MPB, that’s why we are debating, scrutinize.
I am always ready to improve on my technique, modify my technique and learn from others.
Same is expected from you Ironman, rather than you saying you are the best and hence there can be no other technique present or in the future.
Any true regeneration can only be proved by independent monitoring of single, double or triple grafts extracted from donor and implanted with macro and videoscopic pictures of the regenerated grafts at donor and recipient including the petri dish pictures which should show every graft clearly.
We will not consider any telogen hair or failed extractions because we don’t take into consider the same when we do the patch test or in-vivo or in-vitro doubling.
Let me tell you your technique is superior to FUE no doubt but there are other in-vitro, in-vivo Hair Doubling, Hair Tripling, Hair Quadrupling, Stem Cells Hair Multiplication, DP Cells injection is much more superior to yours even today.
You are open to scrutinize independently and compare.
And lastly the link which you have posted is talking about pluripotent stem cell (iPS) and hair follicular stem cells are multipotent and not pluripotent,
and can become hair and skin not anything else and are much safer. Pluripotent stem cell can become neuron, cardiac, etc.
Don’t confuse people with half knowledge.
My present technique is the integration of partial / bisected follicle extraction and with addition of growth factors for repair which used by Histogen and others and autologus serum free stem cells solution used by Aderans, dermal cup sheath, outer root sheath stem cells,
The safety of which is already proven by the 1st phase of clinical trails submited to FDA USA. as first phase of clinical trial is safety trials.
Thanks for giving me access of this article by CD 47 which I will research further and may be incorporate in my technique and this is the power of forums and discussion and debates in the forum.
Don’t you think extracting follicle in-vivo blindly will give rise to multiple extractions, failed extraction, and you will never be perfect every time in extraction where you want to do. Don’t you think my method using fibro optic 0.3 mm wire inside the hollow needle where in I can see the needle where my needle is going and where I am bisecting. In-fact this wire can be used by other HT surgeons.
I am improving everyday in the forum and not sticking to old guns. There will be lot to come after my visit to Edinburg conference of hair research and investigative dermatology where I will meet Jahoda, Dr. Stenn, Dr. Kim, Dr. Fuchs, Dr. Gerd, Dr. Noughton, Dr. Costariles and many others.
The more you know ……..you come to know….. that you do not know enough. This is the sign of wisdom.
Only a empty half vessel …….can be filled up with more knowledge. A vessel is already full with his own ideas and thoughts can never be filled and any new knowledge will be overflows.
Power comes with …….not only from what you know…… but also what you do what you know.
Its when you say you do not know the difference better pluripotent stem cell and multipotent stem cells and bolding making a claim that ------- IS NOT REQUIRED (Stem cells activation and activation). Read the article again. Unfortunately a knowledge about bioengineering, bio-tech, stem cells is half and you pick up article from here and there and tell the forum members about it but not now Ironman because I am here.
Dr. Nigam's bisected hair follicle
IMG]http://www.drnigams.net/images/drgh/DRNCT/Small/1.png[/IMG]
Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic
http://www.drnigams.net/images/drgh/DRNCT/Large/4.png
I had to come to this thread because IM has quoted me and started this thread which is connected to me. I will have to defend my claim.
I hope it is fair for all to have counter argument against the title of this thread. Let discuss whether misleading claim 2 is misleading or is it true?
Preservation medium is an Illusion.
You have given your defense saying that even plucked hair of which you have shown the picture which have follicular stem cells. You are correct there is no dispute.
Counter claim:-
1. Then plucked hair can be used for doubling why to use invasive methods like hollow needles or triple way needles. Dr. Cooley, Dr. Cole have experimented with plucked hair with partial success. We have also started our experiment with addition of stem cells, dp cells, growth factor, ECM and will post the test results next month.
2. Since inherent (you have not done anything to them) stem cells are already present in a plucked follicle / in-vivo bisected follicles & in-vitro bisected follicles including FUT & FUE follicles and since you have already agreed in another thread, that the technical name of this technique is perpendicular partial longitudinal bisection of follicle in-vivo follicular doubling and hence the claims stem cells hair transplant will definitely be consider misleading.
3. The website mentions, that with the help of the needle you extract stem cells and you implant stem cells.
Prove it, which is impossible, in fact you yourself have mentioned in this post that this technique is partial longitudinal follicle bisection and implantation with its inherent stem cells.
Please prove scientifically how the preservation medium multiplies follicle and show any scientific method / article proving that by soaking the graft in any possible culture medium, GF and any other stuff, can multiply follicular stem cells. (It may help in survival and better nutrition).
By the way stem cells in the donor area are already active unlike the stem cells in the MPB recipient bald area.
4. For stem cells to activate you need to loosen the cell membrane with enzymes and special CO2 incubation in a series of process with Growth Factor and proteins in the culture media to keep its tricogensity alive and work on sodium potassium pump on the cell membrane to promote the cell division,
the real stem cell multiplication which is only possible in a FDA certified lab with high sterile conditions like number of organism per 1000 cubic cm in the air of the lab. In normal procedure room or operation theater without laminar air flow, etc one organism is sufficient to spoil the sample of stem cell which is of now use.
Unless you isolate stem cells from the surrounding cells of even the follicle one cannot activate or multiply stem cells. Confirm the same from any independent bio-tech
Next misleading claim according to you:
Let the member and experts decide whether it is a misleading claim or true. This is what you have shown on your website.

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic
That you have bisected the follicle longitudinally and extracted the follicle. But why do we see follicle with bulb in your Petri dish.
We also see transected follicles which you count as 1 instead of 2 follicle per grafts. Why 80% regeneration at donor. Why wastage of some grafts? A follicle with a bulb is like an FUE implantation and recipient will always show growth.
Next claim
You yourself claiming that you give only 80% donor regeneration that means you sacrifice 20% grafts are less at the donor after the procedure.
Now you will have to decide, as I will mention few other technique of donor doubling which will be better.
1. In-vivo hair donor doubling with partial extraction of follicle transverse or longitudinal under the vision of ultra vision or fiber optic camera with addition of GF, ECM, DP Cells, Stem cells. We promise 100% regeneration at donor and 80 to 90 % regeneration at recipient. We can convert nw7 to nw2 in 10 days, ofcourse scarless.
2. Your blind technique of extraction of partial follicular unit longitudinally with wastage of few graft, multiple extraction sites at donor and extraction of follicle with bulb wherein it should have been extraction of follicle as shown in your pictures. Why we should not consider it as a splitting of 2 follicle into 1 as shown in the petri dish. Yes both of us is scarless. you claim 80% donor regeneration and X percentage of recipient regeneration. You can repeat procedure after 6 months and nw7 will takes years to become nw2.
We also offer in-vitro donor doubling wherein we grown 90 follicles from 23 follicle.
Shortly we will also offer pluck hair transplant with addition of stem cell, DP cells, GF to improve on the findings of Dr. Cole, Dr. Cooley.
Next Claim
You yourself say, that you have to use multiple drills or multiple extraction at the donor, We do not use multiple drills or multiple extraction at donor,
Then you decide which technique will be desirable.
Kindly also have a look at different possible types of graft extraction and decide which will be better.
Dr. Nigam's FUE extracted graft

Click the below link to enlarge the above pic
Dr. Nigam's FUT extracted graft

Click the below link to enlarge the above pic
Dr. Nigam's Plucked graft

Click the below link to enlarge the above pic
Please understand we all are here to improve the present technique and get to the cure of MPB, that’s why we are debating, scrutinize.
I am always ready to improve on my technique, modify my technique and learn from others.
Same is expected from you Ironman, rather than you saying you are the best and hence there can be no other technique present or in the future.
Any true regeneration can only be proved by independent monitoring of single, double or triple grafts extracted from donor and implanted with macro and videoscopic pictures of the regenerated grafts at donor and recipient including the petri dish pictures which should show every graft clearly.
We will not consider any telogen hair or failed extractions because we don’t take into consider the same when we do the patch test or in-vivo or in-vitro doubling.
Let me tell you your technique is superior to FUE no doubt but there are other in-vitro, in-vivo Hair Doubling, Hair Tripling, Hair Quadrupling, Stem Cells Hair Multiplication, DP Cells injection is much more superior to yours even today.
You are open to scrutinize independently and compare.
And lastly the link which you have posted is talking about pluripotent stem cell (iPS) and hair follicular stem cells are multipotent and not pluripotent,
and can become hair and skin not anything else and are much safer. Pluripotent stem cell can become neuron, cardiac, etc.
Don’t confuse people with half knowledge.
My present technique is the integration of partial / bisected follicle extraction and with addition of growth factors for repair which used by Histogen and others and autologus serum free stem cells solution used by Aderans, dermal cup sheath, outer root sheath stem cells,
The safety of which is already proven by the 1st phase of clinical trails submited to FDA USA. as first phase of clinical trial is safety trials.
Thanks for giving me access of this article by CD 47 which I will research further and may be incorporate in my technique and this is the power of forums and discussion and debates in the forum.
Don’t you think extracting follicle in-vivo blindly will give rise to multiple extractions, failed extraction, and you will never be perfect every time in extraction where you want to do. Don’t you think my method using fibro optic 0.3 mm wire inside the hollow needle where in I can see the needle where my needle is going and where I am bisecting. In-fact this wire can be used by other HT surgeons.
I am improving everyday in the forum and not sticking to old guns. There will be lot to come after my visit to Edinburg conference of hair research and investigative dermatology where I will meet Jahoda, Dr. Stenn, Dr. Kim, Dr. Fuchs, Dr. Gerd, Dr. Noughton, Dr. Costariles and many others.
The more you know ……..you come to know….. that you do not know enough. This is the sign of wisdom.
Only a empty half vessel …….can be filled up with more knowledge. A vessel is already full with his own ideas and thoughts can never be filled and any new knowledge will be overflows.
Power comes with …….not only from what you know…… but also what you do what you know.
Its when you say you do not know the difference better pluripotent stem cell and multipotent stem cells and bolding making a claim that ------- IS NOT REQUIRED (Stem cells activation and activation). Read the article again. Unfortunately a knowledge about bioengineering, bio-tech, stem cells is half and you pick up article from here and there and tell the forum members about it but not now Ironman because I am here.
Dr. Nigam's bisected hair follicle
IMG]http://www.drnigams.net/images/drgh/DRNCT/Small/1.png[/IMG]
Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic

Click the below link to enlarge the above pic
http://www.drnigams.net/images/drgh/DRNCT/Large/4.png
Comment